Processing of long-stored archival Papanicolaou-stained cytological smears
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چکیده
منابع مشابه
Isolation of DNA from archival Papanicolaou stained cytological smears using a simple salting-out procedure.
DNA from archival Papanicolaou stained and unstained cytological smears was successfully isolated using a simple, rapid and inexpensive salting-out procedure. The quality of DNA was controlled by polymerase chain reaction (PCR) amplification of segments of the human beta-globin, human beta-actin and human papillomavirus L1 genes. Only negligible differences in amplification efficiency were obse...
متن کاملInvestigation into the value of Papanicolaou stained cervical smears for the diagnosis of chlamydial cervical infection.
Forty five (37%) of 121 female contacts of men with non-gonococcal urethritis or gonorrhoea were chlamydia positive, as judged by isolation or by detecting elementary bodies in smears with a fluorescein labelled chlamydial monoclonal antibody. Only six (13%) of these, however, had Papanicolaou stained smears in which there were inclusion like changes suggestive of chlamydial infection. Furtherm...
متن کاملCytological diagnosis of virus-infected cells in Papanicolaou smears and its application in clinical practice.
The cytopathic changes of herpes simplex virus, varicella-zoster virus, the human polyomaviruses, and the trachoma agent can be recognised in smears prepared from a wide variety of specimens. The morphology of virus-infected cells is described, and the clinical value of these observations is discussed. Cytological diagnosis offers a rapid and convenient method of detecting infection with these ...
متن کاملEvaluation of the Papanicolaou-stained cytological smear as a screening technique for asymptomatic gonorrhoea.
To assess the Papanicolaou smear as a screening test for asymptomatic gonorrhoea 311 women who had had simultaneous Papanicolaou smears and cervical cultures performed were studied. Of the 151 women who were culture-positive only 27 (18%) had a positive result by Papanicolaou smear. The presence of either endocervical or metaplastic cells did not increase the frequency with which gonococci were...
متن کاملValue of Papanicolaou-stained smears in the diagnosis of trichomoniasis, candidiasis, and cervical herpes simplex virus infection in women.
In the diagnosis of trichomoniasis Papanicolaou-stained cervical smears gave marginally better results than cultures of vaginal secretion; stained cervical smears and wet films of vaginal material gave similar results. Culture of vaginal secretion on Sabouraud's medium gave the best results in the diagnosis of candidiasis; Papanicolaou-stained smears gave significantly fewer positive results th...
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ژورنال
عنوان ژورنال: British Journal of Cancer
سال: 1996
ISSN: 0007-0920,1532-1827
DOI: 10.1038/bjc.1996.576